Our Publications


K. J. Nieken, K. O'Brien, A. McDonnell, L. Zhaunova and H.Ohkura (2023) A large-scale RNAi screen reveals that mitochondrial function is important for meiotic chromosome organisation in oocytes. Chromosoma 132:1-18. PubMed


C. Repton, C. F. Cullen, M. F. A. Costa, C. Spanos, J. Rappsilber, and H. Ohkura (2022) The phospho-docking protein 14-3-3 regulates microtubule-associated proteins in oocytes including the chromosomal passenger Borealin. PLoS Genetics 18:e1009995. PubMed


P. Barbosa , L. Zhaunova, S. Debilio, V. Steccanella, V. Kelly, T. Ly, and H. Ohkura (2021) SCF-Fbxo42 promotes synaptonemal complex assembly by downregulating PP2A-B56. J Cell Biol. 220: e202009167. PubMed


M. F. A. Costa and H. Ohkura (2019) The molecular architecture of the meiotic spindle is remodelled during metaphase arrest in oocytes. J. Cell Biol. 218: 2854-2864. PubMed

A. Takeda, S. Saitoh, H. Ohkura, K. E. Sawin and G. Goshima (2019) Identification of 15 new bypassable essential genes of fission yeast. Cell Struct Funct 44: 113-119. PubMed


P. Rome and H. Ohkura (2018) A novel microtubule nucleation pathway for meiotic spindle assembly in oocytes. J. Cell Biol. 217: 3431-3445. PubMed

P. Rome and H. Ohkura (2018). Combining microscopy and biochemistry to study meiotic spindle assembly in Drosophila oocytes. Methods Cell Biol. 145: 237-248. PubMed


R. Beaven, R. N. Bastos, C. Spanos, P. Rome, C. F. Cullen, J. Rappsilber, R. Giet, G. Goshima and H. Ohkura (2017) 14-3-3 regulation of Ncd reveals a new mechanism for targeting proteins to the spindle in oocytes. J. Cell Biol. 216: 3029-3039. PubMed


L. Zhaunova, H. Ohkura and M. Breuer (2016) Kdm5/Lid regulates chromosome architecture in meiotic prophase I independently of its histone demethylase activity. PLoS Genet 12: e1006241. PubMed


A. A. Gluszek, C. F. Cullen, W. Li, R. A. Battaglia, S. J. Radford, M. F. Costa, K. S. McKim, G. Goshima and H. Ohkura (2015) The microtubule catastrophe promoter Sentin delays stable kinetochore-microtubule attachment in oocytes. J. Cell Biol. 211: 1113-1120. PubMed Biosights (JCB Podcast)

S. J. Radford, T. L. Hoang, A. A. Gluszek, H. Ohkura and K. S. McKim (2015) Lateral and end-on kinetochore attachments are coordinated to achieve bi-orientation in Drosophila oocytes. PLoS Genet. 11: e1005605. PubMed

M. Breuer and H. Ohkura (2015) A negative regulatory loop within the nuclear pore complex controls global chromatin organization. Genes Dev. 29: 1789-1794. PubMed

Y. Saka, C. V. Giuraniuc and H. Ohkura (2015) Accurate chromosome segregation by probabilistic self-organization. BMC Biology 13: 65. PubMed

R. Beaven, N. S. Dzhindzhev, Y. Qu, I. Hahn, F. Dajas-Bailador, H. Ohkura and A. Prokop (2015) Drosophila CLIP-190 and mammalian CLIP-170 display reduced microtubule plus end association in the nervous system. Mol. Biol. Cell. 26: 1491-1508. PubMed

H. Ohkura (2015) Meiosis: An overview of key differences from mitosis. Cold Spring Harb Perspect Biol. 7: a015859. [Review] PubMed

E. Nikalayevich and H. Ohkura (2015) The NuRD nucleosome remodelling complex and NHK-1 kinase are required for chromosome condensation in oocytes. J. Cell Sci. 128: 566-575. PubMed


S. M. R. Clohisey, N. S. Dzhindzhev and H. Ohkura (2014) Kank is an EB1 interacting protein that localises to muscle-tendon attachment sites in Drosophila. PLoS ONE 9, e106112. PubMed

K. Lesniewska, E. Warbrick and H. Ohkura (2014) Peptide aptamers define distinct EB1- and EB3-binding motifs and interfere with microtubule dynamics. Mol. Biol. Cell. 25: 1025-1036. PubMed


H. M. Syred, J. Welburn, J. Rappsilber and H. Ohkura (2013) Cell cycle regulation of microtubule interactomes: multi-layered regulation is critical for the interphase/mitosis transition. Mol. Cell. Proteomics 12: 3135-3147. PubMed

C. I. Dix, H. C. Soundararajan, N. S. Dzhindzhev, F. Begum, B. Suter, H. Ohkura, E. Stephens and S. L. Bullock (2013) Lissencephaly-1 promotes the recruitment of dynein and dynactin to transported mRNAs. J. Cell Biol. 202: 479-494. PubMed

N. Colombie, A. A. Gluszek, A. M. Meireles and H. Ohkura (2013) Meiosis-specific stable binding of Augmin to acentrosomal spindle poles promotes biased microtubule assembly in oocytes. PLoS Genetics 6: e1003562. PubMed


B. J. Loh, C. F. Cullen, N. Vogt and H. Ohkura (2012) The conserved kinase SRPK regulates karyosome formation and spindle microtubule assembly in Drosophila oocytes. J. Cell Sci. 125: 4457-4462. PubMed

R. F. Reschen, N. Colombie, L. Wheatley, J. Dobbelaere, D. St Johnston, H. Ohkura and J. W. Raff (2012) Dgp71WD is required for the assembly of the acentrosomal meiosis I spindle, and is not a general targeting factor for the gamma-TuRC. Biology Open 1: 422-429. PubMed

J. Zich, A. M. Sochaj, H. M. Syred, L. Milne, A. G. Cook, H. Ohkura, J. Rappsilber and K. G. Hardwick (2012) Kinase activity of fission yeast mph1 is required for mad2 and mad3 to stably bind the anaphase promoting complex. Curr. Biol. 22: 296-301. PubMed


A. M. Meireles, N. S. Dzhindzhev and H. Ohkura (2011) Kebab: kinetochore and EB1 associated basic protein that dynamically changes its localisation during Drosophila mitosis. PLoS ONE 6: e24174. PubMed

R. M. Parton, R. S. Hamilton, G. Ball, L. Yang, C. F. Cullen, W. Liu, H. Ohkura and I. Davis (2011) A Par-1 dependent graded bias in microtubule orientation establishes cell polarity in the Drosophila oocyte. J. Cell Biol. 194: 121-135. PubMed


O. M. Lancaster, M. Breuer, C. F. Cullen, T. Ito and H. Ohkura (2010) The meiotic recombination checkpoint suppresses NHK-1 kinase to prevent reorganisation of the oocyte nucleus in Drosophila. PLoS Genet. 6: e1001179. PubMed


A. M. Meireles, K. H. Fisher, N. Colombie, J. G. Wakefield and H. Ohkura (2009) Wac: a new Augmin subunit required for chromosome alignment but not for acentrosomal microtubule assembly in female meiosis. J. Cell Biol. 184: 777-784. PubMed


N. Colombie, C. F. Cullen, A. L. Brittle, J. K. Jang, W. C. Earnshaw, M. Carmena, K. McKim and H. Ohkura (2008) Dual roles of Incenp crucial to the assembly of the acentrosomal metaphase spindle in female meiosis. Development 135: 3239-3246. PubMed

D. Satoh, A. Sato, T. Tsuyama, M. Saito, H. Ohkura, M. M. Rolls, C. Q. Doe, F. Ishikawa and T. Uemura (2008) Spatial control of branching within dendritic arbors by dynein-dependent transport of Rab5 endosomes. Nature Cell Biol. 10:1164-1171. PubMed

K. Papadopoulou, S. S. Ng, H. Ohkura, M. Geymonat, S. Sedgwick and C. McInerny (2008) Regulation of M-G1 gene expression in fission yeast through Plo1p and forkhead transcription factors. J. Cell Sci. 121: 38-47. PubMed

J. R. Hughes, A. M. Meireles, K. H. Fisher, A. Garcia, P. R. Antrobus, A. Wainman, N. Zitzmann, C. Deane, H. Ohkura and J. G. Wakefield (2008) A microtubule Interactome: complexes with roles in cell cycle and mitosis. PLoS Biol. 6: e98. PubMed


O. M. Lancaster, C. F. Cullen and H. Ohkura (2007) NHK-1 phosphorylates BAF to allow karyosome formation in the Drosophila oocyte nucleus. J. Cell Biol. 179: 817-824. PubMed

R. Basto, F. Gergely, V. M. Draviam, H. Ohkura, K. Liley and J. W. Raff (2007) Hsp90 is required to localise cyclin B and Msps/ch-TOG to the mitotic spindle in Drosophila and humans. J. Cell Sci. 120:1278-1287. PubMed

A. L. Brittle, Y. Nanba, T. Ito and H. Ohkura (2007) Concerted action of Aurora B, Polo and NHK-1 kinases in centromere-specific histone 2A phosphorylation. Exp. Cell Res. 313: 2780-2785. PubMed


Y. Shimada, S. Yonemura, H. Ohkura, D. I. Strutt and T. Uemura (2006) Polarized transport of Frizzled along the planar microtubule arrays in Drosophila wing epithelium. Dev. Cell 10: 209-222. PubMed

D. M. Cottam, J. B. Tucker, M. M. Roger-Bald, J. B. Mackei, J. Macintyre, J. A. Scarborough, H. Ohkura and M. J. Milner (2006) Non-centrosomal microtubule-organising centres in cold-treated cultured Drosophila cells. Cell Mot. Cyt. 63: 88-100. PubMed


C. F. Cullen, A. L. Brittle, T. Ito and H. Ohkura (2005) The conserved kinase NHK-1 is essential for mitotic progression and unifying acentrosomal meiotic spindles in Drosophila melanogaster. J. Cell Biol. 171: 593-602. PubMed

A. R. Page, A. Kovacs, P. Deak, T. Torok T, I. Kiss, P. Dario, C. Bastos, P. Batista, R. Gomes, H. Ohkura, S. Russell, D. M. Glover (2005) Spotted-dick, a zinc-finger protein of Drosophila required for expression of Orc4 and S phase. EMBO J. 24: 4304-4315. PubMed

A. L. Brittle and H. Ohkura (2005) Centrosome maturation: Aurora lights the way to the poles. Curr. Biol. 15: R880-882. [Review] PubMed

S. L. Elliott, C. F. Cullen, N. Wrobel, M. J. Kernan and H. Ohkura (2005) EB1 is essential during Drosophila development and plays a crucial role in the integrity of chordotonal mechanosensory organs. Mol. Biol. Cell. 16: 891-901. PubMed

N. J. Pearson, C. F. Cullen, N. S. Dzhindzhev and H. Ohkura (2005) A pre-anaphase role for a Cks/Suc1 in acentrosomal spindle formation of Drosophila female meiosis. EMBO Rep. 6: 1058-1063. PubMed

N. S. Dzhindzhev, S. L. Rogers, R. D. Vale and H. Ohkura (2005) Distinct mechanisms govern the localisation of Drosophila CLIP-190 to unattached kinetochores and microtubule plus ends. J. Cell Sci. 118: 3781-3790. PubMed

K. C. Slep, S. L. Rogers, S. L. Elliott, H. Ohkura, P. A. Kolodziej and R. D. Vale (2005) Structural determinants for EB1-mediated recruitment of APC and specktraplakins to the microtubule plus end. J. Cell Biol. 168: 587-598. PubMed

A. L. Brittle and H. Ohkura (2005) Mini spindles, the XMAP215 homologue, suppresses pausing of interphase microtubules in Drosophila. EMBO J. 24: 1387-1396. PubMed


N. Reynolds and H. Ohkura (2003) Polo boxes form a single functional domain which mediates interactions with multiple proteins in fission yeast polo kinase. J. Cell Sci. 116: 1377-1387. PubMed

H. Ohkura (2003) Phosphorylation: Polo kinase joins an elite club. Curr. Biol. 13: R912-R914. [Review] PubMed


K. M. May, N. Reynolds, C. F. Cullen, M. Yanagida and H. Ohkura (2002) Polo boxes and Cut23 (Apc8) mediate an interaction between polo kinase and the anaphase promoting complex for fission yeast mitosis. J. Cell Biol. 156: 23-28. PubMed

M. Bownes, B. Charlesworth, I. Davis, D. Finnegan, M Heck, A. Jarman, L. Keegan, H. Ohkura and C. Rabouille (2002) Report on the 17th European Drosophila research conference. Bioessays. 24: 99-101. [Review] PubMed


C. F. Cullen and H. Ohkura (2001) Msps protein is localised to acentrosomal poles to ensure the bipolarity of Drosophila meiotic spindles. Nature Cell Biol. 3:637-642. PubMed

M. M. Donaldson, A. A. M. Tavares, H. Ohkura, P. Deak and D. M. Glover (2001) Metaphase arrest with centromere separation in polo mutants of Drosophila. J. Cell Biol. 153: 663-676. PubMed

H. Ohkura, M. A. Garcia and T. Toda (2001) Dis1/TOG universal microtubule adaptors- one MAP for all? J. Cell Sci. 114: 3805-3812. [Review] PubMed


C. F. Cullen, K. M. May, I. M. Hagan, D. M. Glover and H. Ohkura (2000) A new genetic method to isolate functional interacting genes - high plo1 dependent mutant and their suppressors define genes in mitotic and septation pathways in fission yeast. Genetics 155: 1521-1534. PubMed


C. F. Cullen, P. Deak, D. M. Glover and H. Ohkura (1999) mini spindles: a gene encoding a conserved microtubule associated protein required for the integrity of the mitotic spindle in Drosophila. J. Cell Biol. 146: 1005-1018. PubMed

D. P. Mulvihill, J. Petersen, H. Ohkura, D. M. Glover and I. M. Hagan (1999) Plo1 kinase recruitment to the spindle pole body and its role in cell division in Schizosaccharomyces pombe. Mol. Biol. Cell. 10: 2771-2785. PubMed


H. Ohkura, T. Torok, G. Tick, J. Hoheisel, I. Kiss and D. M. Glover (1997) Mutation of a gene for a Drosophila kinesin like protein, Klp38B, leads to failure of cytokinesis. J. Cell Sci. 110: 945-954.


D. M. Glover, H. Ohkura and A. Tavares (1996) polo kinase: the choreographer of the mitotic stage? J. Cell Biol. 135: 1681-1684. [Review]

H. Ohkura (1996) Roles of polo kinase in the cell division -diversity and conservation-. (in Japanese), Protein, Nucleic acid, Enzyme (Tampakushitsu, Kakusan, Koso) 41: 1863-1868. [Review]


H. Ohkura, I. M. Hagan and D. M. Glover (1995) The conserved Schizosaccharomyces pombe kinase plo1, required to form a bipolar spindle, the actin ring, and septum, can drive septum formation in G1 and G2 cells. Genes & Dev. 9: 1059-1073.


R. E. Mayer-Jaekel, H. Ohkura, P. Ferrigno, N. Andjelkovic, K. Shiomi, T. Uemura, D. M. Glover and B. A. Hemmings (1994) Drosophila mutants in the 55kDa regulatory subunit of protein phosphatase 2A show strongly reduced ability to dephosphorylate substrates of p34cdc2. J. Cell Sci. 107: 2609-2616.


R. E. Mayer-Jaekel, H. Ohkura, R. Gomes, C. Sunkel, S. Baumgartner, B. A. Hemmings and D. M. Glover (1993) The 55 kd regulatory subunit of Drosophila protein phosphatase 2A is required for anaphase. Cell 72: 621-633.

M. Shimanuki, N. Kinoshita, H. Ohkura, T. Yoshida, T. Toda and M. Yanagida (1993) Isolation and characterization of the fission yeast protein phosphatase gene ppe1+ involved in cell shape control and mitosis. Mol. Biol. Cell 4: 303-313.

R. Gomes, R. E. Karess, H. Ohkura, D. M. Glover and C. E. Sunkel (1993) Abnormal anaphase resolution (aar): a locus required for progression through mitosis in Drosophila. J. Cell Sci. 104: 583-593.

R. E. Mayer-Jaekel, H. Ohkura, D. M. Glover and B. A. Hemmings (1993) Protein phosphatase 2A from Drosophila. Adv. Prot. Phosphatases 7: 461-476. [Review]


R. E. Mayer-Jaekel, S. Baumgartner, G. Bilbe, H. Ohkura, D. M. Glover and B. A. Hemmings (1992) Molecular cloning and developmental expression of the catalytic and 65-kDa regulatory subunits of protein phosphatase 2A in Drosophila. Mol. Biol. Cell. 3: 287-298.


H. Ohkura and M. Yanagida (1991) S.pombe gene sds22+ essential for midmitotic transition encodes a leucine-rich repeat protein that positively modulates protein phosphatase-1. Cell 64: 149-157.

N. Kinoshita, H. Yamano, F. Le Bouffant-Sladeczek, H. Kurooka, H. Ohkura, E. M. Stone, M. Takeuchi, T. Toda, T. Yoshida and M. Yanagida (1991) Sister-chromatid separation and protein dephospholylation in mitosis. CSH Sym.Quant.Biol. 56: 621-628.


N. Kinoshita, H. Ohkura and M. Yanagida (1990) Distinct, essential roles of type 1 and 2A protein phosphatases in the control of the fission yeast cell division cycle. Cell 63: 405-415.

H. Ohkura and N. Kinoshita (1990) Mitosis and protein phosphatases (in Japanese), Experimental Medicine Supplement (Jikken igaku Zokango) 8 (no.11): 93-98. [Review]

N. Kinoshita, H. Ohkura and M. Yanagida (1990) Essentiality of protein phosphatases in the cell cycle regulation (in Japanese), Experimental Medicine Monthly (Jikken igaku Gekkan) 8 (no.10): 29-33. [Review]


H. Ohkura, N. Kinoshita, S. Miyatani, T. Toda and M. Yanagida (1989) The fission yeast dis2+ gene required for chromosome disjoining encodes one of two putative type 1 protein phosphatases. Cell 57: 997-1007.


H. Ohkura, Y. Adachi, N.Kinoshita, O. Niwa, T. Toda and M. Yanagida (1988) Cold-sensitive and caffeine-supersensitive mutants of the Schizosaccharomyces pombe dis genes implicated in sister chromatid separation during mitosis. EMBO J. 7: 1465-1473

T. Hirano, H. Ohkura, N. Kinoshita, T. Toda and M. Yanagida (1988) Evidence for the coupling of sister chromatid separation during mitotic anaphase and the decrease of intercellular cAMP concentration in Schizosaccharomyces pombe. In Cell Cycle Control in Eukaryotes (ed. D. Beach, C. Basilico and J. Newport), pp.195-200. Cold Spring Harbor: Cold Spring Harbor Laboratory Press.


T. Uemura, H. Ohkura, Y. Adachi, K. Morino, K. Shiozaki and M. Yanagida (1987) DNA topoisomerase II is required for condensation and separation of mitotic chromosomes in S.pombe. Cell 50: 917-925.

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